Dissemination of Integron Class 1 among XDR isolates of P. aeruginosa in Najaf City

Al-Jelehawy, Alaa Hamza Jaber and Nader, Baker Ghassan and Salah, Khadija Thaer (2023) Dissemination of Integron Class 1 among XDR isolates of P. aeruginosa in Najaf City. South Asian Journal of Research in Microbiology, 17 (4). pp. 19-23. ISSN 2582-1989

[thumbnail of Jelehawy1742023SAJRM111096.pdf] Text
Jelehawy1742023SAJRM111096.pdf - Published Version

Download (288kB)


Background: Integrons are gene capture systems observed in chromosomes, plasmids, and transposons, where identified and capture multiple gene cassettes. Integerons are consists of 3 elements: the initial is integrase gene (int1) encoding integrase enzyme (int1), which is essential to insertion or deletion of specific site. The second element is attI as integron-connected recombination site. The 3rd element is promoter (Pc), which is required in the expression of cassette-connected genes.

Aim of the Study: This thesis designed to examine the occurrence of integron class one genes among XDR isolates of P. aeruginosa.

Methods: An overall of 79 specimens were isolated from patients during the period of study and the specimens were carried to the laboratory. By monoplex PCR assay, the 19 XDR isolates of P. aeruginosa were investigated for the presence of integrons class one using intI1primer.

Results: This result found that 8 isolates are isolated from burns, 7 isolates are from urine, 4 isolates are from wounds. The PCR results show that 17 (89.5%) of the isolates harbored integrase (intI1) gene, while two isolate (10.5%) was negative for intI1 gene.
Conclusions: Present result showed elevated occurrence of integrons class one among XDR isolates of P.aeruginosa.

Item Type: Article
Subjects: Eprints STM archive > Biological Science
Depositing User: Unnamed user with email admin@eprints.stmarchive
Date Deposited: 05 Jan 2024 06:28
Last Modified: 05 Jan 2024 06:28
URI: http://public.paper4promo.com/id/eprint/1751

Actions (login required)

View Item
View Item