Potential therapeutic impacts of phyllanthus and costus aqueous extracts on CcL4 intoxicated rats

Bakground: Chronic liver damage is a worldwide common pathology, characterised by an inflammatory and fibrotic process that leads to a progressive evolution from chronic hepatitis to cirrhosis and hepatocellular carcinoma. A major role of fats and oxidative stress has been recently demonstrated in the pathogenesis of liver diseases. It was a newly introduced plant from Mexico. In India, it is grown in gardens, especially in Kerala, where the fresh leaves are eaten raw by diabetic people Objective: This work aimed to study the effect of phyllanthus and costus aqueous extracts on CcL4 intoxicated rats. Material and methods: Thiry mature male albino rats weighing 150±5g were obtained from Laboratory of Animal Colony, Helwan, Egypt. Rats were divided into 6 equal groups (n=5 rats each); one group was kept as a control negative, while the other 5 groups were injected subcutancosly (s/c) by carbon tetrachloride (CcL4) in parrafin oil 50% VN (2m1 / kg b.wt.) twice a week for two weeks for liver intoxication then , one group of them was left as control positive group (C +ve) while the rest four groups were given orally two doses (250 and 500 mg/kg) of phyllanthus and costus aqueous extracts (250 mg/kg and 500 mg/kg for the group. At the end of experimental period (45 days), blood samples were collected for serum separation to determine serum liver enzymes (AST, ALT and ALP), total cholesterol, triglycerides, atherosclerosis index, lipoprotein fraction (HDLc, LDLc and VLDLc), kidney function(Creatinine, Urea and Uric acid) and histopathological examination for the liver. Results: The obtained results demonstrated that phyllanthus and costus aqueous extracts caused significant improvement in liver enzymes, lipid profiles and kidney functions in CcL4 intoxicated rats. Conclusion: According to the results, phyllanthus and costus aqueous extracts could be tried on intoxicated patieats to improve liver &kidney function