New Copper(II) Complexes of Peptides: DNA Binding and Photocleavage Studies

Sudhamani, C. N. and Naik, H. S. Bhojya and Girija, D. and Aravinda, T. (2011) New Copper(II) Complexes of Peptides: DNA Binding and Photocleavage Studies. International Research Journal of Pure and Applied Chemistry, 1 (2). pp. 42-57. ISSN 22313443

[thumbnail of Published_Naik_2011IRJPAC307.pdf] Text
Published_Naik_2011IRJPAC307.pdf - Published Version

Download (306kB)


Peptides were synthesized by the reaction of Fmoc-amino acid chloride and aminoacid ester hydrochloride in DCM. The obtained peptide ligands were characterized by elemental analysis, NMR, IR and mass spectra. Three copper complexes of peptides Fmoc.Ala-val-OH (F-AVOH), Fmoc-Phe-Leu-Ome (F-PLOMe) and Z-Ala-Phe-CO-NH2 (Z-APCONH2) and have been synthesized and characterized by elemental analysis, IR and mass spectral data. The IR spectral results shows that the –NH group of peptides chain involved in bonding with metal ion. The binding of the complexes with calf thymus DNA have been investigated using absorption spectroscopy, viscosity measurements and thermal denaturation methods. The results suggest that the complexes bound to CT-DNA by partial intercalation with binding constant ≈ 3.9 - 4.8x104 M-1. The photonuclease activities of the complexes were assayed on pUC19 DNA and they exhibit efficient photonuclease properties.

Item Type: Article
Subjects: Eprints STM archive > Chemical Science
Depositing User: Unnamed user with email admin@eprints.stmarchive
Date Deposited: 04 Jul 2023 04:56
Last Modified: 27 Oct 2023 04:55

Actions (login required)

View Item
View Item