Plant Regeneration from Immature Cotyledon derived Cultures of Soybean (Glycine max L. Merr)

High frequency morphogenic calli induction was attained in Glycine max from immature cotyledons. Eleven genotypes and three media were tested for in vitro morphogenic efficiency. The morphogenesis was dependent upon genotype and the explants inoculation medium. Plant regeneration efficiency was highest in genotypes JS 90-41 (53.35%) followed by JS 80-21 (41.0%) when the in vitro culture medium was a combination of 0.5 mg l-1 NAA and 3.0 mg l-1 BAP and contained MS macro and micronutrients, vitamins and agar. Phenotypically normal plants were regenerated from the immature cotyledons explants.