Molecular Detection and Diversity Analysis of Colletotrichum sp from Chilli Using RAPD-PCR

The molecular variability among the isolates of Colletotrichum capsici and Colletotrichum gloeosporioides that differed in virulence was analysed by means of random amplified polymorphic DNA (RAPD) using 10 random primers. Analysis of the genetic coefficient matrix derived from the scores of RAPD profile showed that maximum per cent similarities between species of C. capsici and C. gloeosporioides which were in the range of 92% respectively. Cluster analysis using the unweighted pair-group method with arithmetic average (UPGMA) clearly separated the isolates into two clusters (I and II), confirming the genetic diversity among the isolates of C. capsici and C. gloeosporioides from chilli. RAPD analysis in the present study showed a clear difference between C. gloeosporioides and C. capsici. Furthermore, C. capsici isolates were found to be more closely related than C. gloeosporioides isolates.