Phenol Profiling of Chilli (Capsicum annuum L.) leaves by LC-MS

A lab experiment was conducted at the Department of Biotechnology, Anand Agricultural University, Anand, Gujarat, during 2023. The study focused on phenol profiling of 28 genotypes of chilli along with ACCMS 1 (P1) and ACS 18-08 (P2) using LC-MS. Chilli leaf samples were collected three months after transplanting. A total of 20 phenolic acids were used as standards: salicylic acid, gallic acid, hydroquinone, esculin hydrate, pyrocatechol, methylumbelliferone, umbelliferon, quercetin, coumaric acid, caffeic acid, chlorogenic acid, ferulic acid, cinnamic acid, syringaldehyde, fraxetin, 4-hydroxy cinnamaldehyde, aminobenzoic acid, catechin hydrate, sinapic acid, and epigallocatechin gallate. Among these, six phenolic acids—ferulic acid, caffeic acid, epigallocatechin gallate, sinapic acid, gallic acid, and quercetin—were detected in measurable quantities in the chilli leaves. The result indicated in present study that in parent ACCMS 1 ferulic acid, caffeic acid, epigallocatechin gallate, sinapic acid and gallic acid (0.0075 ppm, 0.0740 ppm, 0.0119 ppm, 0.0083 ppm and 0.1470, respectively) ppm were found lower as compared to parent ACS 18-08. Only one phenolic acid quercetin was only present in ACCMS 1 and not detected in ACS 18-08.