Influence of Rosemary and Garlic on some Haematological parameters and Immunological Functions on Cirrhotic Liver Rats

The study was conducted to investigate the effect of rosemary and garlic supplementation on some haematological parameters and immune functions in cirrhotic liver rats.Fifty two male albino rats weighing 180 ± 5 g were used. The first main group (n=6) was fed on the basal diet during the experimental period and kept as a negative control group. The second main group (n =46) was intraperitoneally injected with CCl4 at a dose of 0.5ml/100 g. body weight for the first time and followed by a dose of 0.3 ml/100 g b.w. twice a week over a period of six weeks in order to induce liver cirrhosis.Fourinjected rats were randomly chosen for pathological examination to ensure that livers become cirrhotic. The other rats (n=42) were divided into 7 subgroups (6 rats each) as follows: Subgroup (1) was fed on basal diet as a positive control group. Subgroups (2 and 3) were fed on basal diet supplemented with 5% and 10 % rosemary respectively. Subgroups (4 and 5) were fed on basal diet supplemented with 5% and 10% garlic respectively. Subgroups (6 and 7) were fed on basal diet supplemented with 5% and 10 % of (rosemary and garlic) at a ratio of (1:1) respectively.Rats were fed the experimental diet for a period of 8 weeks. Results indicatedthat the highest (IgM) and (IgG) levelsin serum were observed in the group fed combination of rosemary and garlic compared to positive control. Diets supplemented with rosemary or garlic or their combination at different levels significantly increased (P<0.05) the mean levels of serum total protein, albumin and globulin respectively, compared to the control positive group. The highest increase in protein parameters concentrations were observed at the groups fed on basal diet supplemented with a mixture of rosemary and garlic. Supplementation with combination of rosemary and garlic showed significant decrease (P<0.05) in the mean serum levels of (AST), (ALT) and (ALP) respectively compared to the control positive group. On the other hand, the supplementation with rosemary, garlic and their combination significantly (P<0.05) increased the mean level of RBCs, WBCs, PCV and Hemoglobin compared to the positive control group. The highest increase in the tested parameters was observed at the groups that fed on a combination of rosemary and garlic.